In this work two different fluorochromes (Alexa 594 and Alexa 680) are conjugated to the same monoclonal antibody (Cetuximab) for obtaining a characteristic M-shaped dual-peak spectrum. Dual-labeling of Cetuximab by mixing both fluorochromes before the conjugation step gives spectral results similar to those of mixing of fluorochrome-labeled Cetuximab after the conjugation step (P>0.05). In conclusion, both methods may be used equivalently for producing a dual-labeled single-antibody probe. Futu
