應用微流體混合器晶片於食品中甲醛含量快速檢測
Rapid Analysis of Formaldehyde in Food Utilizing by
Microfluidic Mixer Chip
研究生:林雅慧 Lin, Ya-Hai
指導教授:劉展冏、傅龍明 Liu, Zhan-Jion g、Fu, Lung-Ming
【摘要】
不當添加甲醛可能會造成食品安全上的問題。微流體晶片(Microfluidic chip) 配合雷射激發螢
光偵測法 (Laser-induced fluorescence detection, LIF)備受矚目。本研究以 4-amino-3-penten-2-one
(Fluoral-P)和甲醛作用 分別以光譜分析儀與螢光光譜儀確定其吸光值範圍與螢光強度 再於 3D
圓形微流體混合器(Microfluidic mixer)行螢光衍生化(Fluorescence derivatization),以雷射激發螢光法
快速偵測。設計四種不對稱管道(1:3,1:5,1:7,1:9)晶片,中間具 micro-chamber 作為微混合器,
先以 10
-5
M Rhodamine B 螢光染劑測試出理想的混合管道設計,以達到良好的混合效果。
結果顯示 全波長掃描之實驗組,隨著甲醛濃度與反應時間的增加,於 410 nm 吸光表現愈顯
著。甲醛行螢光檢測之螢光訊號,隨著濃度的下降其線性度愈佳(R
2
=0.9936)。過高濃度甲醛螢光
衍生物,分子間過多螢光基團訊號集中,而干擾全波長與螢光的偵測,於晶片上則以 50 ppm 以下
來作測定。晶片經混合檢測後,以 1:7 型式管道設計混合效果為佳,甲醛於此晶片中行 LIF 測定,
隨著甲醛濃度的降低,螢光訊號愈不顯著且易出現雜訊,偵測極限只達 0.4 ppm (R
2
=0.9954),而
濃度低於 1 ppm 時,其 S/N 比訊號表現度隨之降低,故無法進一步降低濃度來做檢測。
關鍵字:微流體晶片、雷射激發螢光、螢光衍生化、甲醛、微流體混合器
【Abstract】
Inappropriate additions of formaldehyde in foods may cause food safety problems. Microfluidic
chip electrophoresis with laser-induced fluorescence detection has received growing attention. This study
firstly confirmed the absorption and fluorescence intensity of formaldehyde via fluorescence
derivatization with 4-amino-3- penten-2-one (Fluoral-P), followed by rapid detection of fluorescence
derivatization through an optimal unbalance channel design with three-D circular microfludic mixer.
Design four of unbalance channel chip (1:3, 1:5, 1:7, 1:9), micro-chamber as micro-mixer in middle of
that, detection of the perfection mixer channel design by 10
-5
M Rhodamine B fluorescence reagent to
achieved good mixer efficiency.