关于大鼠中脑导水管周围灰质内 GABA
与阿片 μ 受体共存能神经元的观察
【关键词】 GABA
关键词: GABA;受体，阿片样，μ;水管周灰质;显微镜检查，共焦;免疫组织化
学;大鼠
摘 要:目的 观察中脑导水管周围灰质（PAG）内是否存在 γ-氨基丁酸
（GABA）与阿片 μ 受体（MOR）共存神经元. 方法 免疫荧光组织化学双重染色技
术，染色结果在激光扫描共聚焦显微镜下观察. 结果 GABA 阳性神经元主要见于
PAG 中、尾段内侧区和腹外侧区，阳性神经元多散在分布并多为小型，中型者较少;
散在分布的 MOR 阳性神经元主要见于 PAG 中、尾段的腹外侧区，多为中、小型神经
元;散在并同时呈 GABA 和 MOR 双重染色阳性的中、小型神经元主要见于 PAG 中、尾
段的腹外侧区. 结论 PAG 内有 GABA 与 MOR 共存的神经元，提示内源性阿片物质对
PAG 内的 GABA 能神经元可能有调控作用.
Keywords:GABA;receptors，opioid，mu;periaqueductal gray;microscopy，
confocal;immunohis-tochemistry;rats
Abstract:AIM To observe whetherγ-aminobutyric acid（GABA）
andμopioid receptor（MOR）co-exist in neurons of the periaqueductal
gray（PAG）in the rat.METHODS Im-munofluorescent histochemical double-
staining technique was used.The staining results were observed with
laser scanning confocal microscope.RESULTS GABA-immunoreactive（GABA-
IR）neurons were mainly seen in the medial and ven-trolateral subregions
at the middle and caudal levels of the PAG.Most of the sparsely
distributed GABA-IR neurons were small-sized and the number of medium-
sized GABA-IR neurons was small.Scattered MOR-IR neurons were mainly
observed in the ventrolateral subregion at the middle and cau-dal levels
of the PAG.Most of the MOR-IR neurons were small-and medium-sized.Small-
and medium-sized neurons simultaneously showing both GABA-IR and MOR-IR
were scattered in the ventrolateral subregion at the middle and cau-dal
levels of the PAG.CONCLUSION There are GABA and MOR co-existing neurons
in the PAG.The present results suggest that endogenous opioid-like
substances might regu-late GABAergic neurons in the PAG.
0 引言
以往的研究证明，中脑导水管周围灰质（PAG）内 5-羟色胺（5-HT）能下行投
射系统在调控伤害性信息向中枢的传入过程中发挥着重要作用，而γ-氨基丁酸
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