CRISPR-Cas9基因编辑技术在基因功能和作物育种中的研究进展1.pdf

, CRISPR-Cas9,

,

Zhao Shanshan Di Yihuan Hao Guangfei

technique relies on engineering endonuclease to produce double strand breaks (DSB) at target sites. Subsequently,

double strand breaks were repaired via non-homologous end-joining (NEHJ) or homology-directed repair (HDR),

resulting in mutation and sequence replacement, respectively. At present, the most widely used gene editing techni-

que is CRISPR-Cas9 system, which is composed of Cas9 protein and single-stranded RNA. Under the guidance of

RNA, the target gene is edited. This paper briefly introduces the development of gene editing technology, focusing

on the development of CRISPR-Cas9 system and its latest research progress in gene function and crop breeding.

Gene editing technology, CRISPR-Cas9, Gene function, Crop breeding

CRISPR-

CRISPR-