维普资讯 http://www.cqvip.com
第
21
卷第
6
期
2007
年
12
月
高校化学工程学报
Journal
ofChemical
Engineering
ofChinese
Universities
No.6
Vo
l.
21
De
c.
2007
文章编号:
1003-9015(2007)06-0964-07
神经干细胞对去血清诱导
PC12
细胞凋亡的作用
李香琴
l
，
刘天庆
l
，
朱苦苦
l
，
葛
丹
l
，
崔占峰
2
(1.大连理工大学大连市千细胞与组织工程研友中心，
辽宁大连
116024;
2
.Department
ofEngìneering
Scìence, University
ofOxford
, Parks Road, Oxford
OXl
3PJ
, U K)
摘
要:肾上腺嗜锡细胞瘤细胞
(PCI2
cells)
去血清后，其凋亡与多巳胶能神经元的凋亡有许多共同之处，今通过研究
神经干细胞(N
SCs)
对去血清诱导的
PC12
细胞凋亡的作用，进一步为
NSCs
移植治疗神经系统疾病提供相应的实验和
理论依据。将正常培养的
PC12
细胞与
NSCs
以不同的方式进行去血清共培养，观察
PC12
细胞的形态，检测
PC12
细
胞的活性，计算
PC12
细胞的存活率，同时检测培养基中胶质细胞源神经营养因子
(GDNF)
的浓度，分析不同培养方式
FNSCs
对去血清诱导凋亡的
PC12
细胞的作用以及
NSCs
与去血清诱导凋亡的
PC12
细胞共培养后，其分泌
GDNF
的
能力。结果表明:①去血清诱导
PC12
细胞凋亡呈时间依赖性，去血清
72h
后
.PCI2
细胞存活的比率为
44.25%:
②
NSCs
培养基对去血清诱导凋亡的
PCl2
细胞没有明显的保护作用:③
NSCs
培养上清及
NSCs
对去血清诱导凋亡的
PC12
细
胞具有保护作用:④
NSCs
与去血清诱导凋亡的
PC12
细胞共培养后，分泌
GDNF
的能力增强。
关键诩
g
神经干细胞;
PC12
细胞:神经营养因子:调亡;共培养
中图分类号:
Q813.11
文献标
i
只码
:A
Effect
of
Neural
Stem
Cells
00
Apoptosis
of
PC12
Cells
loduced
by
Serum
Deprivatioo
LI
Xìang-qìn
l
, LIU Tian-qing
l
, ZHU Lei-Iei
l
,
GE
Dan
l
,
CUI
Zhan-feng
2
(1.Dalain Research & Develop Center for Stem Cell and Tissue Engineering, Dalian Unìversity ofTechnology,
Dalian 116024, China; 2.Department
ofEngìneering
Science, Unìversity
ofOxford
, Parks Road, Oxford
OXI
3PJ, U K)
Abstract:
The apoptosìs
of
pheochromocytoma cells lìne
12
(PC
12
cells) induced
by
serum deprivation is
similar to that
of
dopaminergic neuron cells. In this paper, the potentìal cytoprotective effects
of
neural stem
cells (NSCs)
on
the apoptosis
of
PC
12
cells induced by serum deprivation were investigated to provide
experimental and theoretical basis for the therapies
of
nervous system diseases
via
NSCs
transplantation. The
normal PC
12
cells were co-cultured with
NSCs
in DMEMJF
12
medium
仕
ee
of
serum and then PC
12
cell
morphologies
, viabilities and survival were observed with inverted microscope and assessed with CCK-8 assay,
respectively. Besides, the concentrations
of
glial derived neurotrophic factor (GDNF) in different media were
detected with
GDNF
Elisa ki
t.
Finally, the effect
ofNSCs
on
PCI2
cell apoptosis induced
by
serum deprivation
and the
NSCs'
ability to secrete
GDNF
after co-culture were analyzed. The results show
tha
t:(
PC12 cell
apoptosis induced
by
serum deprivation increases with time and after 72 h, only about 44.25% PC
12
cells
survive;
(
NSCs
culture
medium
protects against PC
12
cell apoptosis insignificantly; (
NSCs'
supernatant
and
NSCs
prevent PC12 cells from apoptosis mildly; ( the amount
ofGDNF
secreted by
NSCs
increases after
co-culture the
NSCs
with the apoptotic PC
12
cells induced
by
serum deprivation.
Key
words:
neural stem cells; PC
12
cells; GDNF; apoptosis; co-culture
1
号|
士一同
帕金森病
(Parkinson's
disease,
PD)
是一种慢性神经变性疾病，随着人口老龄化程度的提高，
PD
的发
收稿日期:
2ω
6-
1
0-
30
，修订日期:
2007-03-02.
基金项目:国家自然科学基金
(30670525):
两个基地国际合作项目
(202008):
辽宁省科学技术基金资助项目
(20022140).
作者简介
z
李香琴
(197
忡，女，辽宁大连人.大连理工大学讲师，博士.通讯联系人
z
刘天庆，
E-mail:
liutq@dlu
t.
edu.cn
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