Objective: We study the biomedical optical properties of the color light and near-infrared fluorescence separated-merged imager. Materials and Methods: The color light and near-infrared fluorescence separated-merged imager can illuminate the visible light and the near-infrared light of 760 ± 10 nm, receiving the reflected light and 835 ± 10 nm near-infrared fluorescence, and display the color, fluorescence and merge image. ICG solution of different concentration, including standing time, was all
