Teicoplanin (TCP) is an important lipoglycopeptide antibiotic produced by fermenting Acti-noplanes teichomyceticus. The change in TCP concentration is important to measure in the fermentation process. In this study, a reagent-free and rapid quantification method for TCP in the TCP–Tris–HCl mixture samples was developed using near-infrared (NIR) spectroscopy by focusing our attention on the fermentation process for TCP. The absorbance optimization (AO) partial least squares (PLS) was proposed and
