Adaptive aberration correction in confocal scanning fluorescence...

Adaptive optics is implemented in a confocal scanning fluorescence microscopy using a wavefront sensorless correction scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. We demonstrate the idea of using phantom fluorescence samples experimentally. Enhanced imaging contrast and improved signal level ar