红芪多糖对糖尿病大鼠心肌纤维化的影响及其潜在机制
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中国科技论文在线
红芪多糖对糖尿病大鼠心肌纤维化的影响
及其潜在机制
#
张晓敏
1
，何继瑞
2
，李洁
1
，徐坤
2**
基金项目：甘肃省普通中药科研立项资助课题（GZK-2011-26）
作者简介：张晓敏（1987—），女，硕士研究生，研究方向：糖尿病基础与临床
通信联系人：何继瑞（1963—），男，主任医师，教授，糖尿病基础与临床. E-mail: hjrlzys63@163.com
（1. 兰州大学第二临床医学院，兰州 730000； 5
2. 兰州大学第二医院内分泌与代谢临床医学中心，VIP 糖尿病科，兰州 730030）
摘要：目的 探讨红芪多糖对 2 型糖尿病大鼠心肌纤维化的影响及可能机制。方法 采用高
脂高糖饲料加单次腹腔注射链脲佐菌素（30mg/kg）制备 2 型糖尿病大鼠模型。大鼠分为正
常对照组（NC 组）、糖尿病组（DM 组）及红芪多糖组（HPS 组）3 组，每组 10 只，干预10
8 周后所有大鼠称重，计算心重指数。实时荧光定量 PCR 法（Real-time quantitative PCR）
测定心肌局部 TGF-β1、Smad3、Smad7 mRNA 水平，免疫组化法测定 TGF-β1、Smad3、
Smad7 蛋白水平，透射电镜下观察大鼠心肌超微结构，Masson 染色测定心肌胶原含量。结
果 ①与 NC 组比较，DM 组、HPS 组心肌胶原含量增多，间质纤维化明显，H/B、TGF-β
1、Smad3 表达均明显升高，Smad7 表达降低（P＜0.01）；与 DM 组比较，HPS 组大鼠心肌15
纤维化减轻，H/B、TGF-β1、Smad3 表达下降，Smad7 表达有所升高（P＜0.05）。②心肌
病理改变：与 NC 组相比，DM 组大鼠心肌胶原纤维明显增多，排列紊乱，线粒体肿胀；而
HPS 干预组上述改变减轻。结论 红芪多糖通过抑制 TGF-β1/Smads 信号通路，延缓了糖
尿病大鼠心肌纤维化的发生和发展。
关键词：2 型糖尿病；红芪多糖；心肌纤维化；转化生长因子β1；smad3；smad7 20
中图分类号：R587.1
Effect of Hedysarum Polybotys Saccharide on myocardial
fibrosis in diabetic rats
Zhang Xiaomin
1
, HE Jirui
2
, Li Jie
1
, Xu Kun
2
25
(1. School of Second Clinical Medicine of Lanzhou University,Lanzhou 730000;
2. The Endocrinology and Metabolism Center,The VIP Diabetes Department of the Second
Hospital of Lanzhou University,Lanzhou 730030)
Abstract: Objective To investigate the effect of Hedysarum Polybotys Saccharide（HPS） on
myocardial fibrosis in diabetic rats and its underlying mechanism. Methods Type 2 diabetic 30
animal models was established by feeding high-sucrose and high-fat diet and intraperitoneal
injecting low dose streptozotocin（30mg/kg）.After the success of modeling,diabetic rats were
divided into three groups:normal control group（NC group）,diabetes group（DM group）and
Hedysaryum Polybotys Saccharide group（HPS group）. There are ten rats in each group.After 8
weeks，we weighted all rats and calculated the heart body weight ratio(H/B),Real-time quantitative 35
PCR was used to estimate the levels of TGF-β1,Smad3,Smad7 mRNA in mycardium. The
expression of myocardial TGF-β1,Smad3,Smad7 protein was determined by
immunohistochemistry. Myocardial pathologic changes were observed under transmission
electronic microscope.Masson staining was used to detect the volume fraction of myocardial
collagen.Rerults ①The levels of H/B,the expression of TGF-β1 and Smad3 in DM group and 40
HPS group were significantly higher than those in NC group,but levels of Smad7 were lower than
NC group（P＜0.01）；the levels of H/B, the expression of TGF-β1 and Smad3 in HPS group were
lower than those in DM group,the expression of Smad7 was higher than DM group（P＜
0.05）.②The pathological changes of myocardial ultrastructure：In DM group,collagen fibrils were
increased and arranged disorderly, mitochondria swelled,while these changes of myocardial 45
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