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物联网-智慧传输-基于双频调相的荧光传感系统的研究与实验分析.pdf
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物联网-智慧传输-基于双频调相的荧光传感系统的研究与实验分析.pdf
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摘 要
在学术研究或者工业上,通常需要经常监测
pH
(酸碱度)和
2
pO
(氧分压)
这两个参数。在医学方面,
pH
和
2
pO
对评估危重病人的生命特征具有重要作用,
常用于临床诊断病人血气、体液和呼吸活动等等;在食物加工业,对
pH
和
2
pO
量的控制直接关系到饮用水的质量、食物的新鲜程度和食物或饮料的含菌量;对
于重工业,
pH
值是废水处理和环境酸雨的监控的重要指标;在海洋分析和研究
领域,同样需要密切关注这两个参数。
目前,多参量光纤传感器依然存在较多问题。在制作方面,使用多个互不相
关的传感器整合到一个探头的手段,不仅使得制作难度加大,而且使得传感探针
体积庞大,导致每个独立传感单元所处空间不一致,空间分辨率变差;在传感机
理方面,每个独立传感器需要不同测量方法和条件,因而需要进行不同的光路和
电路的设计,造成结构复杂且难有通用性。
本课题鉴于当前多参量光纤传感技术的存在问题,尝试设计一款新型传感系
统,将传感单元置于一个传感探针中,通过光相位调制方式测量不同的两种物质
浓度。鉴于对称型分子如氧分子,其结构只有伸缩振动而不存在偶极矩变化的振
动,所以无法使用光吸收原理进行直接测量。荧光测量技术应运而生并因其高灵
敏度和灵活的选择性成为当前众多领域的研究对象。
本文利用荧光相位调制方式将测量光强信息转为相位信息,从而简化设计结
构;在传统双荧光寿命参比技术基础上,寻找两种不同的荧光物质,均在同一激
发波长下存在吸收峰和发射波长下存在相似发射峰,而氧分子和氢离子充当荧光
猝灭剂,在不同浓度下引起相应荧光产生幅度和相位上的改变。结合数学模型,
推出新的荧光寿命和荧光强度比计算方法,并通过对传感系统模块化仿真分析,
设计可行方案和预测实验可能产生的问题。
最后,结合上述分析,设计传感系统硬件平台,分别对气体氧浓度、溶解氧
浓度和 pH 缓冲液三种环境下测量氧或氧与 pH 双参量,并尝试通过拟合的方式
得到相关浓度与相位、荧光寿命比的关系,现象基本符合理论猜想。总之,本文
希望借着对双参测量技术仿真与实验,能起到一个抛砖引玉的效果。
关键词:荧光分析法,光相位调制法,双荧光寿命参比技术,双参荧光光纤传感
器
万方数据
II
Abstract
In academic or industrial research usually need frequent measurement about pH
and oxygen partial pressure. In medicine, pH or oxygen partial pressure plays an
important role to evaluate critically ill patient's life characteristics. They commonly
used in patients with clinical diagnosis of blood, body fluids and respiratory activity,
etc. In the food processing industry, the amount of H+ and O
2
is directly related to the
quality of drinking water, food freshness and bacterial content of food or drink. For
heavy industry, the value of pH is an important indicator in wastewater treatment and
environmental monitoring acid rain. In ocean analysis and research, also need to pay
close attention to these two parameters.
At present, multi-parameter optical fiber sensors still have many problems. In the
aspect of production, to turn multiple unrelated sensor into a integrated probe not only
makes production more difficult, but also makes the sensing probe bulky. Therefore,
the spatial resolution gets worse because each sensor unit in the space is not consistent.
In terms of sensing mechanism, each individual sensor need different measurement
methods and measurement conditions, thus different design of optical paths or circuits
which will result in complicated structure and difficult versatility for every sensor
become more necessary.
In view of the fact that reliability of measurement in multi-parameter optical fiber
sensing technology remains few problems, it is excited to design a new sensing
system, which arranges all sensing unit in a probe using different optical modulation
to measure two kinds of concentration. Research shows that the symmetric molecules
such as oxygen molecules, their structure determines they have only stretching
vibration but no vibration produced by change of dipole moment, so that we cannot
use optical absorption principle for direct measurement. Fluorescence measurement
technology emerges as the times require and become the object of study in many
fields because of its high sensitivity and high selectivity.
In this paper, firstly, we measure phase information instead of light intensity
information which simplifies the design structure. According to the traditional double
fluorescence lifetime reference technology, we need to find two different fluorescent
substances which have the similar emission wavelength and the similar absorption
wavelength. When these two fluorescent substances are excited by the frequency
modulated light, they can emit the same frequency but larger wavelength light called
万方数据
III
fluorescence. Oxygen and hydrogen ion acts as a fluorescence quencher and cause the
amplitude and phase of corresponding fluorescence changing. In basis of The
fluorescence decay model, we promoted a new calculation method and simulate with
Simulink to analyze the design scheme and predict the possible problems.
Finally, base on the above analysis, we design a sensor system experimental
platform. We measured oxygen concentration in the gas, dissolved oxygen and the
value of pH in buffer solution. We use Matlab or 1stotp to fit the relationship between
concentration, phase and fluorescence lifetime. The experimental phenomenon is
consist of theoretical conjecture. In a word, this paper hopes to promote the
multi-parameter measurement technology and the analysis show us a good beginning.
Keywords: Fluorescence analysis method, Optical phase modulation method, Dual
fluorescence lifetime reference technology, Double parameter fluorescence optical
fiber sensor
万方数据
目 录
摘 要............................................................................................................................ I
Abstract ......................................................................................................................... II
第 1 章 绪论................................................................................................................. 1
1.1 课题概述......................................................................................................... 1
1.1.1 课题来源............................................................................................... 1
1.1.2 课题背景和研究意义........................................................................... 1
1.2 国内外研究现状............................................................................................. 2
1.2.1 光纤传感器研究现状........................................................................... 2
1.2.2 生物医学领域下光纤传感器研究现状............................................... 3
1.2.3 光纤传感器的发展趋势....................................................................... 6
1.3 本文主要研究内容......................................................................................... 7
1.4 本文结构......................................................................................................... 7
第 2 章 荧光分析法原理............................................................................................. 9
2.1 荧光发光原理与特征..................................................................................... 9
2.2 荧光寿命与荧光猝灭................................................................................... 10
2.2.1 荧光寿命............................................................................................. 10
2.2.2 荧光猝灭机理..................................................................................... 11
2.3 荧光分析法............................................................................................ 13
2.4 本章小结....................................................................................................... 18
第 3 章 传感系统原理与仿真................................................................................... 19
3.1 数字频率合成器........................................................................................... 19
3.1.1 频率合成器原理................................................................................. 19
3.3 数字锁相放大器........................................................................................... 22
3.2.1 相关检测的锁定放大器原理.............................................................. 23
3.2.2 锁相放大仿真..................................................................................... 26
3.3 双荧光寿命参比测量与改进....................................................................... 30
3.3.1 双荧光寿命参比原理......................................................................... 31
3.3.2 改进型双荧光寿命参比原理............................................................. 31
3.4 本章小结....................................................................................................... 35
第 4 章 传感系统设计............................................................................................... 36
4.1 系统总体框架............................................................................................... 36
4.2 发光电路设计........................................................................................ 37
万方数据
4.2.1 硬件设计............................................................................................. 37
4.2.2 软件设计............................................................................................. 41
4.3 光信号采集设计........................................................................................... 44
4.4 本章小结....................................................................................................... 51
第 5 章 实验结果与数据分析................................................................................... 52
5.1 实验仪器和材料........................................................................................... 52
5.2 空气氧浓度检测与分析............................................................................... 52
5.3 溶解氧浓度检测与分析............................................................................... 54
5.4 缓冲液 pH 与氧分压的测定 ........................................................................ 55
5.5 本章小结....................................................................................................... 56
第 6 章 总结与展望................................................................................................... 57
6.1 全文工作总结............................................................................................... 57
6.2 未来的工作展望........................................................................................... 58
致 谢......................................................................................................................... 59
参考文献..................................................................................................................... 60
万方数据
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