Manual PowerMarker V3.0 - 2 -
TABLE OF CONTENTS
Chapter 1: Introduction....................................................................................................... 4
1.1 What is PowerMarker? ............................................................................................. 4
1.2 Data types handled by PowerMarker........................................................................ 4
1.3 Methods implemented in PowerMarker.................................................................... 4
1.4 System requirements................................................................................................. 5
1.5 Installing and uninstalling PowerMarker.................................................................. 5
1.6 How to cite PowerMarker......................................................................................... 5
1.7 Acknowledgements................................................................................................... 5
1.8 Upgrade PowerMarker.............................................................................................. 5
Chapter 2: Tutorial.............................................................................................................. 6
2.1: Creating a project..................................................................................................... 6
2.2: Importing a dataset................................................................................................... 7
2.3: Choosing a subset from the dataset.......................................................................... 9
2.4: Producing a table of summary statistics .................................................................. 9
Chapter 3: Data manipulation........................................................................................... 11
3.1 Data import ............................................................................................................. 11
3.1.1 Import dataset................................................................................................... 11
3.1.2 Import frequency data...................................................................................... 12
3.1.3 Import distance data......................................................................................... 13
3.1.4 Import tree data................................................................................................ 13
3.1.5 Import table data.............................................................................................. 13
3.1.6 Import text data................................................................................................ 14
3.2 Dataset manipulation .............................................................................................. 14
3.2.1 Export dataset................................................................................................... 14
3.2.2 Choose subset................................................................................................... 14
3.2.3 Partition dataset................................................................................................ 14
3.2.4 Attach/detach marker information................................................................... 14
3.2.5 Attach/detach assay information...................................................................... 14
Chapter 4: Data analysis ................................................................................................... 15
4.1 Summary statistics.................................................................................................. 15
4.1.1 Basic statistics.................................................................................................. 15
4.1.2 Allele and genotype frequencies...................................................................... 16
4.1.3 Haplotype frequencies ..................................................................................... 16
4.1.4 Hardy-Weinberg disequilibrium...................................................................... 18
4.1.5 Two-Locus Linkage disequilibrium................................................................. 19
4.1.6 Multi-Locus linkage disequilibrium ................................................................ 20
4.2 Design ..................................................................................................................... 20
4.2.1 Line selection................................................................................................... 20
4.3 Structure analysis.................................................................................................... 20
4.3.1 Population differentiation test.......................................................................... 20
4.3.2 Classical F-statistics......................................................................................... 20
4.3.3 Population specific F-statistics ........................................................................ 21
4.3.4 Coancestry matrix............................................................................................ 21
4.4 Phylogenetic analysis.............................................................................................. 21