关于 HPLC-ELSD 法测定银杏叶提取物
及其制剂中 银杏内酯 A、B、C 和白果内
酯含量
【摘要】 目的 建立 HPLC-ELSD 法测定银杏叶提取物（extract of
Ginkgo biloba，EGb）及其制剂中银杏内酯 A、B、C 及白果内酯含量。方法 以
Kromasil C18 为固定相，甲醇-四氢呋喃-水（1∶24∶75）为流动相，流速0.8
ml/min；柱温：35℃；蒸发光散射检测器蒸发温度为100℃，气体流速为 2.5
L/min。结果 银杏内酯 A、B、C、白果内酯的定量限分别为 0.106、0.104、
0.105、0.105 mg/ml，银杏内酯 A、B、C、白果内酯分别在 0.127～0.506、0.129
～0.548、0.131～0.576、0.131～0.561 mg/ml 浓度范围内其峰面积与浓度的线性
关系良好（r＞0.999），方法回收率为 92.4% ～100.8%，日内精密度及日间精密
度的变异系数分别小于 2%、3%。结论 HPLC-ELSD 法简单、准确，为银杏叶提取物
中内酯类成分的质量控制提供了依据。
【关键词】 高效液相色谱 蒸发光散射检测器 银杏叶提取物 含量测定
Abstract： Objective To establish a high performance liquid
chromatography (HPLC) with evaporative light-scattering detector (ELSD)
to determine the content of ginkgolide A， B， C and bilobalide in the
extract of Ginkgo biloba (EGb) leaves and its pharmaceutical agents.
Methods Kromasil C18 was designated as the stationary phase and methanol
to tetrahydrofuran to water (1∶24∶75) as the mobile phase; with the
column temperature (250mm 4.6mm， 5μm) of 35℃ at a flow rate of 0.8
ml/min， the evaporation temperature of 100℃ and the flow rate of gas
of 2.5 L/min. Results The limits of quantitation for ginkgolide A， B，
C and bilobalide were 0.106， 0.104， 0.105 and 0.105 mg/ml，
respectively. This method is linear over concentration ranges of 0.127
～ 0.506， 0.129 ～ 0.548， 0.131 ～ 0.576 and 0.131 ～ 0.561 mg/ml for
ginkoglide A， B， C and bilobalide， respectively (r>0.999). The
recoveries for ginkgolide A， B， C and bilobalide were 92.4%～100.8%.
The coefficient of variation of intraday and interday precision was
respectively less than 2% and 3% for all compounds. Conclusion The
validated method is accurate and easy to handle， thus providing the
basis for the quality control of EGb.
Key words: HPLC; ELSD; extract of Ginkgo biloba leaves;
determination
银杏叶为银杏科植物银杏（Ginkgo biloba L.）的干燥叶，其提取物是经现代
提取工艺从银杏叶中提取的活性物质，其主要活性化学成分为两大类：黄酮类及萜
类［1］。银杏内酯是二萜类和倍半萜类内酯，二萜类内酯主要有银杏内酯 A 、
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