USER MANUAL
MINI DISSOLVED OXYGEN PROBE (MINI-DO)
Specifications
Dimensions dia 15mm, length 90mm
Measuring principle galvanic cell, self temperature compensating
Type % saturation
Output c. 25 mV at 100% O
saturation, output impedance is c. 2 kOhm
Wiring White wire is zero (0 or GND,) brown wire is positive
Flow requirements typically 1 cm/sec, depending on oxygen saturation and temp
Cable 3 m, other lengths available on request
Measuring range 0-200%
Accuracy typically better than ± 1% of measured value, depending on calibration
Repeatability typically better than ± 0.5% of measured value
Response time T
(90% of end value) is <20 sec
Maintenance
Between sample measurements, immerse the tip of the probe in distilled water. For long-time
storage keep the probe in the moist storage chamber to avoid evaporation of the electrolyte
fluid.
Calibrating the probe
1. Connect the probe to an oxygen analyzer.
2. Place the probe in a calibration chamber with a water saturated atmosphere or in a
mixed air-equilibrated water sample.
3. When the reading stabilizes, adjust the meter reading according to the chart in table 1,
following the meter manufacturer’s instructions. If measuring in a saline solution,
remember to correct for changes in oxygens solubility in water according to tabulated
values, i.e. Green&Carrit, 1967. Journal of Marine Biology 25:140-147 (appendix).
4. Next, place the tip of the probe in oxygen free distilled water. Oxygen free distilled
water can be achieved by purging nitrogen gas into the sample water or by dissolving
approximately 10 grams of Na
2
SO
3
in 500 mL of distilled water.
5. When the reading is stable, adjust the zero control on the meter until the reading
displayed indicates zero.
6. Rinse the probe with distilled water.
7. Place the probe into the sample, and record the sample dissolved oxygen when the
reading is stable.