Expression, Purification and Characterization of extramacrochaetae (emc) from the silkworm

家蚕extramacrochaetae（emc）基因的克隆、表达和特征分析，杨庆，曾凤辉，Extramacrochaetae蛋白是螺旋环螺旋超家族中的一个蛋白，在果蝇的发育过程中起到非常重要的调控作用。我们成功的在大肠杆菌中表达了家�

Purification and Characterization of a novel β-galactosidase from the newly isolated strain Erwinia rhapontici E602

Erwinia rhapontici E602株β-半乳糖苷酶的分离纯化及性质研究，姜轶珂，夏雨，从冻土中分离得到一种产β-半乳糖苷酶的菌株E602，经鉴定后为大黄欧文氏菌。该菌株在26℃培养并以乳糖诱导其产β-半乳糖苷酶。得到的

Cloning, Purification and Characterization of NADP+-dependent malic enzyme from the oleaginous fungus Mortierella alpina

高山被孢霉中NADP依赖型苹果酸酶的克隆、纯化和酶学性质研究，杨佳玉，胡欣洁，苹果酸酶是一种氧化脱羧酶，它催化L-苹果酸脱羧生成丙酮酸和二氧化碳，同时将NAD(P)+还原成NAD(P)H。在高山被孢霉中，NADP依赖型的苹果�

Purification，Spectroscopic and Thermodynamic Characterization of Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia

金属β-内酰胺酶L1的表达纯化、光谱表征和热力学研究，高会洲，闫小艳，为了深入研究抗生素耐药问题，本文介绍了在大肠杆菌中表达来源于嗜麦芽寡养单胞菌的金属β-内酰胺酶L1，利用离子交换色谱进行纯化�

Isolation, Purification and Identification of Torularhodin from Sporidiobolus pararoseus by Column Chromatography and its Anti-Cancer Effect on LNCaP Cells

Sporidiobolus pararoseus中红酵母红素的柱色谱分离纯化鉴定及其对LNCaP细胞增殖的影响，杜超，施秋妤，本实验建立了一种通过柱色谱从Sporidiobolus pararoseus发酵产物中分离纯化红酵母红素的方法。通过高效液相色谱（HPLC）鉴定证明色素的纯�

Fusion Expression and Purification of Escherichia coli Oligopeptidase B

大肠杆菌寡肽酶B的融合表达及纯化，闫健斌，江雪源，大肠杆菌寡肽酶B(OpdB)以前被称作蛋白酶Ⅱ，具有类胰蛋白酶的特异性，可以在赖氨酸和精氨酸处切割多肽，它属于新丝氨酸蛋白酶中的�

Expression and purification of NSH2 domain protein for NMR research

NSH2蛋白的原核表达纯化和同位素标记，牛皋，郭江峰，SHP-2蛋白是一种含有两个SH2结构域的磷酸酶。SHP-2的N端SH2结构域(NSH2)是细胞因子或病毒因子激活该蛋白的分子内靶点。将NSH2以不同的方法

Separation, purification, and production of apigenin from celery with ultrasonic-assisted beta-glucosidase hydrolysis

用超生波葡萄糖苷酶裂解法从芹菜中分离,纯化和制备芹菜素，张谦，周鸣鸣，芹菜素，作为一种重要的食品类化合物，存在于许多种类的蔬菜中。越来越多的证据表明，吸食芹菜素，对许多疾病的预防起到了很好的

Isolation and Purification of ATP Synthase in Mitochondria

牛心线粒体ATP合成酶的分离纯化的方法学研究，朱杰，孙润广，科研工作者们在过去的50年前赴后继的工作中深入了ATP合成酶的功能，并努力尝试解析该酶的空间结构以其能够从结构基础上对ATP合成酶�

Soluble expression and two-step purification of the cytoplasmic domains of Arabidopsis CORYNE in Escherichia coli

拟南芥CORYNE蛋白胞内激酶区在大肠杆菌中的表达与纯化研究，屈玮，赵珩，CORYNE (CRN)是一个受体类似蛋白激酶，由胞内激酶区和跨膜区组成，通过与CLAVATA2互作，参与调控拟南芥干细胞分化。我们采用原核系统在�

FYCHWQDE-based affinity chromatography of IgG: Effect of ligand density and purification from human serum

八肽FYCHWQDE亲和色谱柱纯化人血清中人抗体：配基密度的影响，薛爱英，余林玲，八肽FYCHWQDE已被证明可作为人抗体（hIgG）的亲和配基，本文将研究FYCHWQDE亲和色谱介质配基密度对其动态结合行为的影响及其对从人血清�

[book] The Essentials: understanding nanoscience and nanotechnology

Book (Pradeep2007) Pradeep, T. (ed.) The Essentials: understanding nanoscience and nanotechnology McGraw-Hill, 2007 Abstract: 1. Introduction—The Canvas of Nano 3 1.1 Nano and Nature 3 1.2 Our Technologies and the World We Live in 5 1.3 Nano—The Beginning 9 Review Questions 12 References 12 PART TWO Experimental Methods 2. Investigating and Manipulating Materials in the Nanoscale 15 2.1 Introduction 15 2.2 Electron Microscopies 20 2.3 Scanning Probe Microscopies 43 2.4 Optical Microscopies for Nanoscience and Technology 54 2.5 Other Kinds of Microscopies 59 2.6 X-Ray Diffraction 75 2.7 Associated Techniques 81 Review Questions 81 References 82 Additional Reading 84 For more information about this title, click here   PART THREE Diversity in Nanosystems 3. Fullerenes 89 3.1 Introduction 89 3.2 Discovery and Early Years 91 3.3 Synthesis and Purification of Fullerenes 94 3.4 Mass Spectrometry and Ion/Molecule Reactions 95 3.5 Chemistry of Fullerenes in the Condensed Phase 96 3.6 Endohedral Chemistry of Fullerenes 99 3.7 Orientational Ordering 100 3.8 Pressure Effects 101 3.9 Conductivity and Superconductivity in Doped Fullerenes 102 3.10 Ferromagnetism in C60.TDAE 103 3.11 Optical Properties 103 3.12 Some Unusual Properties 104 Review Questions 105 References 106 Additional Reading 112 4. Carbon Nanotubes 114 4.1 Introduction 114 4.2 Synthesis and Purification 117 4.3 Filling of Nanotubes 119 4.4 Mechanism of Growth 120 4.5 Electronic Structure 120 4.6 Transport Properties 122 4.7 Mechanical Properties 122 4.8 Physical Properties 123 4.9 Applications 123 4.10 Nanotubes of Other Materials 124 Review Questions 125 References 126 Additional Reading 127  5. Self-assembled Monolayers 128 5.1 Introduction 128 5.2 Monolayers on Gold 129 5.3 Growth Process 138 5.4 Phase Transitions 141 5.5 Patterning Monolayers 142 5.6 Mixed Monolayers 144 5.7 SAMS and Applications 144 Review Questions 153 References 154 Additional Reading 155 6. Gas Phase Clusters 156 6.1 Introduction 156 6.2 History of Cluster Science 158 6.3 Cluster Formation 158 6.4 Cluster Growth 161 6.5 Detection and Analysis of Gas Phase Clusters 163 6.6 Types of Clusters 166 6.7 Properties of Clusters 172 6.8 Bonding in Clusters 176 Review Questions 177 References 177 Additional Reading 178 7. Semiconductor Quantum Dots 179 7.1 Introduction 179 7.2 Synthesis of Quantum Dots 182 7.3 Electronic Structure of Nanocrystals 187 7.4 How Do We Study Quantum Dots? 189 7.5 Correlation of Properties with Size 194 7.6 Uses 195 Review Questions 197 References 198 Additional Reading 198  8. Monolayer-protected Metal Nanoparticles 199 8.1 Introduction 199 8.2 Method of Preparation 200 8.3 Characterization 200 8.4 Functionalized Metal Nanoparticles 204 8.5 Applications 206 8.6 Superlattices 208 Review Questions 212 References 213 Additional Reading 214 9. Core-shell Nanoparticles 215 9.1 Introduction 215 9.2 Types of Systems 216 9.3 Characterization 225 9.4 Properties 227 9.5 Applications 234 Review Questions 240 References 241 Additional Reading 243 10. Nanoshells 244 10.1 Introduction 244 10.2 Types of Nanoshells 245 10.3 Properties 252 10.4 Characterization 255 10.5 Applications 257 Review Questions 259 References 259 Additional Reading 260 PART FOUR Evolving Interfaces of Nano 11. Nanobiology 263 11.1 Introduction 263 11.2 Interaction Between Biomolecules and Nanoparticle Surfaces 264  11.3 Different Types of Inorganic Materials Used for the Synthesis of Hybrid Nano-bio Assemblies 269 11.4 Applications of Nano in Biology 271 11.5 Nanoprobes for Analytical Applications—A New Methodology in Medical Diagnosis and Biotechnology 276 11.6 Current Status of Nanobiotechnology 278 11.7 Future Perspectives of Nanobiology 280 Review Questions 280 References 281 Additional Reading 282 12. Nanosensors 283 12.1 Introduction 283 12.2 What is a Sensor? 284 12.3 Nanosensors—What Makes Them Possible? 285 12.4 Order from Chaos—Nanoscale Organization for Sensors 285 12.5 Characterization—To Know What has been Put In 288 12.6 Perception—Nanosensors Based on Optical Properties 289 12.7 Nanosensors Based on Quantum Size Effects 291 12.8 Electrochemical Sensors 293 12.9 Sensors Based on Physical Properties 294 12.10 Nanobiosensors—A Step towards Real-time Imaging and Understanding of Biological Events 296 12.11 Smart Dust—Sensors of the Future 298 Review Questions 299 References 299 Additional Reading 300 13. Nanomedicines 301 13.1 Introduction 301 13.2 Approach to Developing Nanomedicines 302 13.3 Various Kinds of Nanosystems in Use 303 13.4 Protocols for Nanodrug Administration 305 13.5 Nanotechnology in Diagnostic Applications 307 13.6 Materials for Use in Diagnostic and Therapeutic Applications 310 13.7 Future Directions 312 Review Questions 313  References 313 Additional Reading 315 14. Molecular Nanomachines 316 14.1 Introduction 316 14.2 Covalent and Non-covalent Approaches 317 14.3 Molecular Motors and Machines 318 14.4 Molecular Devices 319 14.5 Single Molecule Devices 320 14.6 Practical Problems with Molecular Devices 327 Review Questions 328 References 328 Additional Reading 329 15. Nanotribology 330 15.1 Introduction 330 15.2 Studying Tribology at the Nanoscale 331 15.3 Nanotribology Applications 337 15.4 Outstanding Issues 341 Review Questions 342 References 342 Additional Reading 343 PART FIVE Society and Nano 16. Societal Implications of Nanoscience and Nanotechnology 347 (in Developing Countries) 16.1 Introduction 348 16.2 From the First Industrial Revolution to the Nano Revolution 349 16.3 Implications of Nanoscience and Nanotechnology on Society 351 16.4 Issues—An Outlook 353 16.5 Nano Policies and Institutions 358 16.6 Nanotech and War—Nano Arms Race 360 16.7 Public Perception and Public Involvement in the Nano Discourse 360 16.8 Harnessing Nanotechnology for Economic and Social Development 362  16.9 Conclusions 368 Review Questions 368 References 369 Additional Reading 371

handbook of industrial crystallization

Crystallization is an important separation and purification process used in industries ranging from bulk commodity chemicals to specialty chemicals and pharmaceuticals. In recent years, a number of environmental applications have also come to rely on crystallization in waste treatment and recycling processes

Solubilization and refolding with simultaneous Purification of Recombinant Human Granulocyte Colony-stimulating Factor expressed by Escherichia coli as Inclusion Bodies

大肠杆菌表达的重组人粒细胞集落刺激因子的溶解和复性并同时纯化，王超展，王骊丽，对大肠杆菌表达的包涵体形式的重组人粒细胞集落刺激因子(rhG-CSF)进行了溶解和纯化。研究了pH和脲对rhG-CSF包涵体溶解的影响，结果表明

Effect of purification, dehydration, and coagulation processes on the optical parameters of biological tissues

In this study, the effects of purification, dehydration, and coagulation processes on the absorption and reduced scattering coefficients of chicken liver tissues have been investigated by using a single integrating sphere system. The purification process performed on the tissue samples to remove blood residue has been found to cause a slight change in the optical parameters. Although the dehydration process brings about an increase in the absorption coefficient due to the water loss, no direct r

Antibody Purification

chromatography purification

SUMS04 Elements of Logic via Numbers and Sets, D. L. Johnson (1998).pdf

Springer Undergraduate Mathematics Series(SUMS)

High-efficiency AlGaN/GaN/AlGaN tunnel junction ultraviolet light-emitting diodes

AlGaN is the material of choice for high-efficiency deep UV light sources, which is the only alternative technology to replace mercury lamps for water purification and disinfection. At present, however, AlGaN-based mid- and deep UV LEDs exhibit very low efficiency. Here, we report a detailed investigation of the epitaxy and characterization of LEDs utilizing an AlGaN/GaN/AlGaN tunnel junction structure, operating at

Synthesis of high-purity silver nanorods with tunable plasmonic properties and sensor behavior

Through anisotropic Ag overgrowth on the surface of Au nanobipyramids (AuNBPs), high-purity and sizecontrolled Ag nanorods (Au/AgNRs) are obtained by a simplified purification process. The diameters of the Au/AgNRs are determined by the size of the as-prepared AuNBPs, and the lengths of the Au/AgNRs are tunableusing different amounts of Ag precursor in the growth solution. Surface-enhanced Raman scattering (SERS) studies using Rhodamine-6G (R6G) as a test molecule indicate that the Au/AgNRs have