Isolation, Purification and Identification of Torularhodin from Sporidiobolus pararoseus by Column Chromatography and its Anti-Cancer Effect on LNCaP Cells

Sporidiobolus pararoseus中红酵母红素的柱色谱分离纯化鉴定及其对LNCaP细胞增殖的影响，杜超，施秋妤，本实验建立了一种通过柱色谱从Sporidiobolus pararoseus发酵产物中分离纯化红酵母红素的方法。通过高效液相色谱（HPLC）鉴定证明色素的纯�

Isolation and characterization of ten novel polymorphic microsatellites loci in the Chinese pond turtle (Mauremys reevesii) and cross-species amplification in other Cryptodira species

乌龟十个新微卫星位点的分离鉴定及在其它曲颈龟类中的跨物种扩增，刘罗，聂刘旺，Ten polymorphic microsatellite loci were developed for the Chinese Pond turtle (Mauremys reevesii) from (AC)n and (GATA)n microsatellite-enriched genomic library. We screened 32 wi

Isolation and Characterization of Homolog PtLEAFY promoter in Trifoliate Orange (Poncirus trifoliata L. Raf. )

枳PtLEAFY 启动子分离与功能鉴定，梅丽，张金智，柑橘从播种到开花需经历很长的童期，过长的童期给柑橘的遗传育种带来了很大的麻烦。由于PtLEAFY是控制植株开花的关键基因，通过分�

Isolation and Preliminary Identification of Aluminium-resistant Microorganisms from the Rhizophric Soil of Tea Plant

Isolation and Preliminary Identification of Aluminium-resistant Microorganisms from the Rhizophric Soil of Tea Plant，吴颖，梁月荣，Microorganism isolated from rhizospheric soil of tea plant was inoculated in culture medium containing aluminium 5-20 mmol/L and an aluminium-resistant fungus ALF-1 was isolated. T

Isolation and Identification of Glycopeptides from β-Conglycinin hydralysates and their effects on Proliferation of Mice Splenocytes

β-伴大豆球蛋白酶解物中分离鉴定糖肽及其对小鼠脾细胞增殖效果的研究，张雪梅，郭顺堂，Hydrolysates A and P were derived from β-Conglycinin hydrolyzed by Alcalse and Pronase respectively, and then analyzed by gel chromatography of Sephadex G-25. Chromatography patte

Isolation and Characterization of Total N-linked Glycans from Glycoproteins by Ultrafiltration Units and Mass Spectrometry

基于超滤膜辅助的糖蛋白全N-连接糖链的分离和质谱鉴定，杨刚龙，马恬然，【目的】建立一种基于超滤膜分离血清中糖蛋白全N-连接糖链，并利用质谱技术对糖链结构进行分析的方法。【方法】根据糖蛋白及其糖�

Isolation, identification and characterization of human intestinal bacteria with the ability to utilize chloramphenicol as the sole source of carbon and energy

人体肠道中氯霉素同化菌的分离鉴定与特性研究，赵鑫，田丰伟，采用传统微生物分离方法，以氯霉素为培养基唯一碳源，从健康人粪便样品中分离到五株氯霉素同化微生物。根据菌株形态、生理生化特

Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae

羊草LcSAP基因的克隆及提高转基因酵母耐盐性的分析，刘晶莹，杨睎喆，本文通过RACE技术从羊草中克隆LcSAP基因，该基因编码161氨基酸残基，蛋白分子量17.6 kDa。RT-PCR分析表明LcSAP基因在叶中比根中表达量高，�

Isolation and Characterization of Soybean Straw Lignin by Using LiCl/DMSO Solvent System

基于LiCl/DMSO全溶体系的大豆秸秆木质素分离与表征，刘祝兰，曹云峰，提取大豆秸秆酶解木素（CEL）过程中，在酶解之前创新性地使用LiCl/DMSO溶剂体系预处理球磨后原料，从而提高木素提取效率且不引起木素

Isolation and Characterization of a Phytase Gene (Sphy1) from Soybean (Glycine max (L.) Merr.)

大豆(Glycine max (L.) Merr.)植酸酶基因Sphy1 的鉴定及其分子特征研究，郭丽，王娇娇，在萌发的大豆(Glycine max (L.) Merr. cv. Kefeng6) 子叶中，克隆了1个新型大豆植酸酶基因Sphy1。Sphy1的The cDNA全长为 1644 bp，编码 547 个氨基酸残基

Isolation and expression of a new high molecular weight glutenin subunit gene at the Glu-D-1-2 locus from Aegilops tauschii

粗山羊草中定位在Glu-D-1-2的一个新的高分子量谷蛋白亚基的克隆与表达，张艳贞，安学丽，通过SDS-PAGE 和 MALDI-TOF-MS都鉴定了在粗山羊草中两个新的高分子量谷蛋白亚基1Dy12.1*t和1Dy12.2t的迁移率，它们的迁移率均是1Dy12.2t>1Dy12.1*t>1D

PCR and magnetic bead-mediated target capture for the isolation of short interspersed nucleotide elements in fishes

PCR和磁珠介导的靶标捕获法分离鱼类基因组的短散布元件，刘东，朱国利，短散布元件（SINEs）是一类反转座子，广泛分布在各种生物基因组中。同一基因组内存在以不同方向排列的多份拷贝。在生物进化历史上�

Cisco组播管理 To provide you with a thorough understanding of the tools and facilities available for managing, monitoring and fault isolation of IP Multicast networks333

To provide you with a thorough understanding of the tools and facilities available for managing, monitoring and fault isolation of IP Multicast networks333

Principles And Strategies Of Design Patterns

The Principles and Strategies of<br/>The Principles and Strategies of<br/>Design Patterns <br/>Design Patterns <br/>Alan Shalloway, Net Objectives, alshall@netobjectives.com <br/>James R.Trott, Millennium Relief & Development Services, jtrott@mrds.org <br/>(Chapter 14 of the Second Edition of their book: (R)¨Design Patterns Explained: A New<br/>Perspective on Object-Oriented Design? due out in August, 2004) <br/>Design patterns do not exist in isolation, but work in concert with other design <br/>patterns to help you create more robust applications. In this book, you will gain a <br/>solid understanding of twelve core design patterns and a pattern used in <br/>analysis. You will gain enough of a foundation that you will be able to read the <br/>design pattern literature, if you want to, and possibly discover patterns on your <br/>own. Most importantly, you will be better equipped to create flexible and <br/>complete software that is easier to maintain. <br/>

Using the Process Schematic in Plant-wide Disturbance Analysis

This article describes how isolation and diagnosis of the root cause of a plant-wide disturbance is enhanced when process connectivity is considered alongside the results of data-driven analysis. A prototype software has been designed and implemented which, when given an electronic process schematic of a plant and results from a data-driven analysis of process measurements, allows the user to pose queries about the plant and to find root causes of plant-wide disturbances. The plant topology information is written in XML according to the Computer Aided Engineering Exchange (CAEX) schema.

Signal Integrity Effects in Custom IC and ASIC Designs_[R Singh][Wiley][2002]

Signal Integrity is no longer an issue for microprocessor and radio frequency designers who designed high-end custom IC designs. As technology scales, clock frequencies rise, and the need for higher integration of analog and digital IP blocks increases, unwanted parasitic effects become more and more a gating factor to IC design project success. Many of these effects can be prevented or analyzed and fixed in isolation, but increasingly they are becoming highly dependent on each other. This all creates a lot of confusion and subjective opinion in the IC design world about how to deal with these new problems. The purpose of this book is to help clear these confusions, and offer a number of methods for IC designers and CAD engineers to model, and then solve, these problems.

Design and Architecture of CockroachDb

Cockroach is a distributed key:value datastore which supports ACID transactional semantics and versioned values as first-class features. The primary design goal is global consistency and survivability, hence the name. Cockroach aims to tolerate disk, machine, rack, and even datacenter failures with minimal latency disruption and no manual intervention. Cockroach nodes are symmetric; a design goal is homogeneous deployment (one binary) with minimal configuration. Cockroach implements a single, monolithic sorted map from key to value where both keys and values are byte strings (not unicode). Cockroach scales linearly (theoretically up to 4 exabytes (4E) of logical data). The map is composed of one or more ranges and each range is backed by data stored in RocksDB (a variant of leveldb), and is replicated to a total of three or more cockroach servers. Ranges are defined by start and end keys. Ranges are merged and split to maintain total byte size within a globally configurable min/max size interval. Range sizes default to target 64M in order to facilitate quick splits and merges and to distribute load at hotspots within a key range. Range replicas are intended to be located in disparate datacenters for survivability (e.g. { US-East, US-West, Japan }, { Ireland, US-East, US-West}, { Ireland, US-East, US-West, Japan, Australia }). Single mutations to ranges are mediated via an instance of a distributed consensus algorithm to ensure consistency. We’ve chosen to use the Raft consensus algorithm. All consensus state is stored in RocksDB. A single logical mutation may affect multiple key/value pairs. Logical mutations have ACID transactional semantics. If all keys affected by a logical mutation fall within the same range, atomicity and consistency are guaranteed by Raft; this is the fast commit path. Otherwise, a non-locking distributed commit protocol is employed between affected ranges. Cockroach provides snapshot isolation (SI) and serializable snapshot isolation (SSI) semantics, allowing externally consistent, lock-free reads and writes--both from a historical snapshot timestamp and from the current wall clock time. SI provides lock-free reads and writes but still allows write skew. SSI eliminates write skew, but introduces a performance hit in the case of a contentious system. SSI is the default isolation; clients must consciously decide to trade correctness for performance. Cockroach implements a limited form of linearizability, providing ordering for any observer or chain of observers. Similar to Spanner directories, Cockroach allows configuration of arbitrary zones of data. This allows replication factor, storage device type, and/or datacenter location to be chosen to optimize performance and/or availability. Unlike Spanner, zones are monolithic and don’t allow movement of fine grained data on the level of entity groups. A Megastore-like message queue mechanism is also provided to 1) efficiently sideline updates which can tolerate asynchronous execution and 2) provide an integrated message queuing system for asynchronous communication between distributed system components.

The Practice of Cloud System Administration pdf

“There’s an incredible amount of depth and thinking in the practices described here, and it’s impressive to see it all in one place.” —Win Treese, coauthor of Designing Systems for Internet Commerce The Practice of Cloud System Administration, Volume 2, focuses on “distributed” or “cloud” computing and brings a DevOps/SRE sensibility to the practice of system administration. Unsatisfied with books that cover either design or operations in isolation, the authors created this authoritative reference centered on a comprehensive approach. Case studies and examples from Google, Etsy, Twitter, Facebook, Netflix, Amazon, and other industry giants are explained in practical ways that are useful to all enterprises. The new companion to the best-selling first volume, The Practice of System and Network Administration, Second Edition, this guide offers expert coverage of the following and many other crucial topics: Designing and building modern web and distributed systems Fundamentals of large system design Understand the new software engineering implications of cloud administration Make systems that are resilient to failure and grow and scale dynamically Implement DevOps principles and cultural changes IaaS/PaaS/SaaS and virtual platform selection Operating and running systems using the latest DevOps/SRE strategies Upgrade production systems with zero down-time What and how to automate; how to decide what not to automate On-call best practices that improve uptime Why distributed systems require fundamentally different system administration techniques Identify and resolve resiliency problems before they surprise you Assessing and evaluating your team’s operational effectiveness Manage the scientific process of continuous improvement A forty-page, pain-free assessment system you can start using today

Apache Hadoop 3.x state of the union and upgrade guidance

Apache Hadoop YARN is the modern distributed operating system for big data applications. It morphed the Hadoop compute layer to be a common resource-management platform that can host a wide variety of applications. Many organizations leverage YARN in building their applications on top of Hadoop without repeatedly worrying about resource management, isolation, multitenancy issues, etc. The Hadoop Distributed File System (HDFS) is the primary data storage system used by Hadoop applications. It employs a NameNode and DataNode architecture to implement a distributed file system that provides high-performance access to data across highly scalable Hadoop clusters. Wangda Tan and Wei-Chiu Chuang the current status of Apache Hadoop 3.x—how it’s used today in deployments large and small, and they dive into the exciting present and future of Hadoop 3.x—features that further strengthen Hadoop as the primary resource-management platform and the storage system for enterprise data centers. They explore the current status and the future promise of features and initiatives for both YARN and HDFS of Hadoop 3.×. For YARN 3.x, there is powerful container placement, global scheduling, support for machine learning (Spark) and deep learning (TensorFlow) workloads through GPU and field-programmable gate array (FPGA) scheduling and isolation support, extreme scale with YARN federation, containerized apps on YARN, support for long-running services (alongside applications) natively without any changes, seamless application/services upgrades, powerful scheduling features like application priorities, intra-queue preemption across applications, and operational enhancements including insights through Timeline Service v2, a new web UI, better queue management, etc. Also, HDFS 3.0 announced GA for erasure coding, which doubles the storage efficiency of data and thus reduces the cost of storage for enterprise use cases. HDFS added support for multiple standby NameNodes for better availability. For better reliability of metadata and easier operations, Journal nodes have been enhanced to sync the edit log segments to protect against rolling failures. Disk balancing within a DataNode was another important feature added to ensure disks are evenly utilized in a DataNode, which also ensures better aggregate throughput and prevents from lopsided utilization if new disks are added or replaced in a DataNode. The HDFS team is currently driving the Ozone initiative, which lays the foundation of the next generation of storage architecture for HDFS where data blocks are organized in storage containers for higher scale and handling of small objects in HDFS. The Ozone project also includes an object store implementation to support new use cases. And you’ll leave with all the knowledge of how to upgrade painlessly from 2.x to 3.x to get all the benefits.