Gene Transfer
|
Electroporation
... for Better Results
Use the Gene Pulser MXcell
™
electroporation system to quickly optimize conditions for delivering
molecules ef ficiently into mammalian cells — especially into primary and difficult-to-transfect cells.
This high-throughput system uses d isposable 12-, 24-, and 96-well plates and can be used for
transfecting siRNA, plasmid DNA, and other molecules directly into the nucleus.
OptimizeYourResearchTime
■
Use a preset optimiza t ion prot ocol or a gradient protocol to decrease programming time
■
Process an entire multiw ell plat e in 2 minutes or less
Optimize Your Reading Time
■
Take adv antage of Bio-Rad’s extensive library of electroprot ocols
Optimize Your Resources
■
Usefewercellsandlesssample—siRNAorDNA—bydefining
electroporation parameters
Optimize Your Experimental Conditions
■
Programupto24electroporationprotocols,withreplicates,onasingleplate
Optimize Your Bench Space
■
Perform high- t hroughput screening and standard laboratory-scale experiments
Qualify f or an electroporation buffer sample online, or submit your optimization protocol
for a free gift. To learn more, go to www.bio-rad.com/ad/MXcell/
I Optimized...
To find your local sales office, visit www.bio-rad.com/contact/
In the U.S., call toll free at 1-800-4BIORAD (1-800-424-6723)
Visit us on the Web at discover.bio-rad.com
Canc er Endocrinology Immunology Proteases Neuroscience
Development Stem Cells SignalTransduction Glyc obiology
®
Selection expanding weekly—visit www.RnDSystems.com to sign up for weekly new product updates.
For research use only. Not for use in diagnostic procedures.
USA & Canada R&D Systems,Inc. Tel: (800) 343-7475 info@RnDSystems.com
Europe R&D Systems Europe,Ltd. Tel: +44 (0)1235 529449 info@RnDSystems.co.uk
China R&D Systems China Co., Ltd. Tel: (21) 52380373 info@RnDSystemsChina.com.cn
Tools for Cell Biology Research
™
NOW AVAILABLE !
OFFERING MORE THAN
11, 000
QUALITY PRODUCTS
for Cell Biology Research!
Request a catalog online:
www.RnDSystems.com/go/Catalog
GE Healthcare
When it comes to life sciences, GE Healthcare is setting the
standard. T ens of thousands of scientists in ov er 100 countries
around the world rely on our products every day. We have
delivered more than 60 000 research protein purification systems,
1500 BioProcess
™
systems and 12 000 BioProcess columns
worldwide. Our Amersham family of consumables, with its 60-year
heritage, is trusted to provide accurate results time and time again.
But we’re never content to stand still. We constantly strive for
new innovations for tomorrow’s research and drug development.
And the result is groundbreaking products like the ÄKTAdesign
™
platform, IN Cell Analyzer, Ad-A-Gene Vectors, and MabSelect
™
media. Thanks to our technological achievements and global
presence, we’re able to help y ou turn your scientific ideas into
reality – bringing science to life and helping transform healthcare.
We call it Life Science Re-imagined.
Discover more at www.gelifesciences.com
Bringing
science to life
GE Healthcare Bio-Sciences AB, a General Electric Company.
Björkgatan 30, 751 84 Uppsala, Sweden.
© 2007 General Electric Company - All rights reserved.
GE34-07
www.sciencemag.org SCIENCE VOL 319 25 JANUARY 2008
373
CONTENTS
CONTENTS continued >>
DEPARTMENTS
379 Science Online
381 This Week in Science
387 Editors’ Choice
388 Contact Science
391 Random Samples
393 Newsmakers
422 AAAS News & Notes
486 AAAS Meeting Program
497 New Products
498 Science Careers
COVER
A male lark bunting in the Pawnee National
Grassland, Colorado. The plumage quality
of the males determines their reproductive
success, but different aspects of the black and
white markings are preferred by females in
different years. This variability alters the
long-term sexual selection dynamics and
may favor the evolution of multiple sexual
ornaments. See page 459.
Photo: Bruce Lyon
EDITORIAL
385 Solutions for Nigeria
by Rita R. Colwell and Michael Greene
406
LETTERS
Antarctica Invaded A. Ricciardi 409
A Closer Look at the IPCC Report S. Solomon,
R. Alley, J. Gregory, P. Lemke, M. Manning
Response M. Oppenheimer et al.
CORRECTIONS AND CLARIFICATIONS 410
BOOKS ET AL.
Vienna in the Age of Uncertainty 412
Science, Liberalism and Private Life
D. R. Coen, reviewed by M. D. Laubichler
Musicophilia Tales of Music and the Brain 413
O. Sacks, reviewed by J. Phillips-Silver
EDUCATION FORUM
Application of Bloom’s Taxonomy Debunks the 414
“MCAT Myth”
A. Y. Zheng, J. K. Lawhorn, T. Lumley, S. Freeman
PERSPECTIVES
Lining Up to Avoid Bias 416
A. Rokas
>> Report p. 473
Enlightening Rhythms 417
O. Lipan
>> Report p. 482
The Rise and Fall of a Great Idea 418
A. Meibom
>> Report p. 453
Structural Nanocomposites 419
Y. Dzenis
Adaptive Composites 420
R. Vaia and J. Baur
ASSOCIATION AFFAIRS
Science and Technology for Sustainable Well-Being 424
J. P. Holdren
Volume 319, Issue 5862
413
NEWS OF THE WEEK
Dust Storm Rising Over Threat to Famed Rock Art 394
in Utah
A Plan to Capture Human Diversity in 1000 Genomes 395
Max Planck Accused of Hobbling Universities 396
France Launches Public Health School 397
à l’Anglo-Saxonne
SCIENCESCOPE 397
Got Data Questions? NSF’s Indicators Has (Most of) 398
the Answers
“Little” Cosmic Ray Observatory Aims to Make 400
a Big Mark
Where Has All the Stardust Gone? 401
>> Report p. 447
Dutch Universities Split Over Nobel Laureate’s 401
Rehabilitation
NEWS FOCUS
A Time War Over the Period We Live In 402
Why We’re Different: Probing the Gap Between 404
Apes and Humans
Shell Shock Revisited: Solving the Puzzle of 406
Blast Trauma
