微纳传感器原理Principles of Electronic Nanobiosensors

Course Objectives This course will provide an in-depth analysis of the origin of the extra-ordinary sensitivity, fundamental limits, and operating principles of modern nanobiosensors. The primary focus will be the physics of biomolecule detection in terms of three elementary concepts: response time, sensitivity, and selectivity. And, we will use potentiometric, amperometric, and cantilever-based mass sensors to illustrate the application of these concepts to specific sensor technologies. Students of this course will not learn how to fabricate a sensor, but will be able to decide what sensor to make, appreciate their design principles, interpret measured results, and spot emerging research trends. Who Should Take the Course Engineers, chemists, physicists, technology developers, and product managers who have an interest in the emerging field of nanobiosensing. Prerequisites Freshman/sophomore level preparation in physics, chemistry, biology, and mathematics. Course Outline Unit 1: Introduction to Nanobiosensors/ Settling Time L1.1: What are Nanobiosensors, Anyway? L1.2: Basic Concepts: Biomolecules, Analyte Density, Diffusion Distances L1.3: Basic Concepts: Types of Biosensors, Geometry of Biosensing L2.1: Shape of a Surface L2.2: Classical Sensors I L2.3: Classical Sensors II Unit 2: Setting Time L2.4: Sensors with Complex Geometry L2.5: Beating the Limits – Barcode Sensors L2.6: Beating the Limits – Droplet Evaporation L2.7: Beating the Diffusion Limit – Enhanced Diffusion and Fluid Flow L2.8: First Passage and Narrow Escape Time I L2.9: First Passage and Narrow Escape Time II Unit 3: Sensitivity L3.1: Nanobiosensors Sensitivity and Types of Biosensors L3.2: Potentiometric Sensors: Charge Screening for a Planar Sensor L3.3: Potentiometric Sensors: Charge Screening for Cylindrical Sensors L3.4: Potentiometric Sensors: ISFET as a pH-Meter L3.5: Potentiometric Sensors: Why are Biomolecules Charged? L3.6: How to Beat Screening Unit 4: Selectivity L3.7: Amperometric Sensors – Glucose Sensor I L3.8: Amperometric Sensors: Glucose Sensors II L3.9: Amperometric Sensors: Beating the Diffusion Limit by Nanogap Amperometry L3.10: Cantilever-based Sensors: BasicOperation L3.11: Cantilever-based Sensors: Static Response L3.12: Cantilever-based Sensors: Nonlinear Sensing – Flexure FET Unit 5: Putting the Pieces Together L4.1: Introduction and Molecular Recognition L4.2: Physics of Sequential Adsorption L4.3: When all else fails, tag, filer and amplify L4.4: Noise Tranducers L5.1: Genome Sequencer I L5.2: Genome Sequencer II L5.3: Genome Sequencer III L5.4: Concluding Thoughts